Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Kobs/[I] = 107 M_-¹ sec_-¹ as inhibitor of thrombin

Primary TargetThrombin

General description

Extremely potent and selective irreversible inhibitor of thrombin (K_obs/[I] = 10⁷M^-1S^-1). Reacts with thrombin in a 1:1 stoichiometry. Can also be used to inhibit tissue plasminogen activator. Factor VIIa, and Factor XIa.

Extremely potent and selective irreversible inhibitor of thrombin (K_obs/[I] = 10⁷M^-1S^-1). Reacts with thrombin in a 1:1 stoichiometry. Can also be used to inhibit tissue plasminogen activator, Factor VIIa, and Factor XIa. Also available as a 25 mM solution in H₂O (Cat. No. 508773).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Rosado, J. A., et al. 2002. J. Biol. Chem.277, 42157.Odake S., et al. 1995. J. Enzyme Inhib.9, 17.Arni, R.K., et al. 1993. Biochemistry 32, 4727.Yamashita, T., et al. 1993. Thromb. Res. 69, 93.Schmaier, A.H., et al. 1992. Thromb. Res.67, 479.Collen, D., et al. 1982. J. Lab. Clin. Med.99, 76.Kettner, C., and Shaw, E. 1981. Methods Enzymol.80, 826.Kettner, C., and Shaw, E. 1979. Thromb. Res.14, 969.

Packaging

Packaged under inert gas

5, 25 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions (pH ﹤4) are stable for up to 4 months at -20°C. Rapidly decomposes at an alkaline pH.

Sequence

H-D-Phe-Pro-Arg-chloromethylketone, 2HCl

Warning

Toxicity: Standard Handling (A)